BD 558049 - can be used for lysing red blood cells and for fixing leukocytes in one step
allowing investigators to rapidly inactivate kinases
phosphatases and/or proteases
so that the in vivo phosphorylation state of the cell can be examined
BD Phosflow™ Lyse/Fix Buffer is intended to be used for the lysing and fixing of whole blood for use with intracellular flow cytometry
Has been reported to preserve the light scattering properties of the cell
May be used on human and mouse whole blood
Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
In many cases
phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however
not all cell surface markers
however
are compatible with the BD Phosflow Lyse/Fix buffer
