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Propidium Iodide (PI) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis
PI binds to double stranded DNA by intercalating between basepairs
but is excluded from cells with intact plasma membranes
PI can be used in FL3 for inviability exclusion
but should be analyzed in FL2 when used as a counterstain for FITC Annexin V
For Research Use Only
