Western Blot Educational Kit Edvotek™ 6 blots, 2.5 hours Time Range, Refrigerated

Features

• Proteins are separated on a polyacrylamide gel and are transferred (blotted) to a nylon membrane Membrane is exposed to solutions containing primary antibody
• followed by a secondary antibody coupled to an enzyme Membrane is then soaked in a substrate solution to develop the color reaction to identify the antigen protein band Molecular weights of the visible bands are measured using prestained protein markers of known molecular weight Experiment times: Electrophoresis
• one hour
• blot
• overnight
• detection
• two hours and 30 minutes Includes: Instructions
• negative control
• all samples and antibodies
• various reagents and buffers
• membrane and filter paper